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Titlebook: Methods of Protein Separation; Nicholas Catsimpoolas Book 1975 Springer Science+Business Media New York 1975 amino acid.biological.biology

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书目名称Methods of Protein Separation
编辑Nicholas Catsimpoolas
视频video
丛书名称Biological Separations
图书封面Titlebook: Methods of Protein Separation;  Nicholas Catsimpoolas Book 1975 Springer Science+Business Media New York 1975 amino acid.biological.biology
描述This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience prov
出版日期Book 1975
关键词amino acid; biological; biology; development; enzyme; enzymes; experiment; ferritin; nucleic acid; protein; pr
版次1
doihttps://doi.org/10.1007/978-1-4757-1257-5
isbn_ebook978-1-4757-1257-5
copyrightSpringer Science+Business Media New York 1975
The information of publication is updating

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Immunodiffusion,ies. Immunodiffusion tests are performed in semisolid media, usually gels, to allow reactants to diffuse while preventing convective mixing, and to support accumulation of the antigen–antibody reaction product, usually a precipitate, for observation.
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Purification of Chemically Modified Proteins,ques have made chemical modifications of proteins a major tool in protein chemistry (Hirs and Timasheff, 1972.; Means and Feeney, 1971). But, even with the availability of modern procedures, purification and analysis of the chemically modified products still remain as formidable tasks in using chemical modification in protein chemistry.
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Isoelectric Focusing in Polyacrylamide Gel,al and preparative purposes. Finally, IEF defines an important parameter, the isoelectric point (p.), which gives information not only about the composition and conformation of macromolecules but also allows a rational approach to further experimental manipulations.
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Book 1975y, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience prov
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ns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience prov978-1-4757-1257-5
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