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Titlebook: Methods in Protein Sequence Analysis; Proceedings of the 7 Brigitte Wittmann-Liebold Conference proceedings 1989 Springer-Verlag Berlin Hei

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书目名称Methods in Protein Sequence Analysis
副标题Proceedings of the 7
编辑Brigitte Wittmann-Liebold
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图书封面Titlebook: Methods in Protein Sequence Analysis; Proceedings of the 7 Brigitte Wittmann-Liebold Conference proceedings 1989 Springer-Verlag Berlin Hei
描述"Methods in Protein Sequence Analysis - 1988" - contains selected contributions on modern protein- analytical techniques as presented by speakers at the Seventh International Conference on "Methods in Protein Sequence Analysis", held from July 3rd to July 8th, 1988 in Berlin. The book contains information on new methodologies for sensitive amino acid analysis, N- and C-terminal sequence analysis, and protein and peptide purification. In addition recent mass spectrometric approaches are described, as an alter­ native technique to the common stepwise degradative sequence analysis of polypeptides by the Edman method. The book presents new possibilities in the design of sequencers and sophisticated equipment for the structural analysis of peptides and proteins. It describes practical approaches for the investigation of protein domains and protein complexes, and contains review chapters on the crystallization of cell organelles as well as on recent theoretical aspects of protein folding mechanisms. The nature of protein folding is not yet understood, but further advances in this area would greatly enhance our present knowledge of protein structure and function. Further, the book gives e
出版日期Conference proceedings 1989
关键词Amino acid; Peptide; primary structure; proteins; sequence analysis
版次1
doihttps://doi.org/10.1007/978-3-642-73834-0
isbn_softcover978-3-642-73836-4
isbn_ebook978-3-642-73834-0
copyrightSpringer-Verlag Berlin Heidelberg 1989
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A Comparison of 9-Fluorenylmethylchloroformate (FMOC-Cl) Amino Acid Analysis with Ninhydrin and Phenalysis technology to keep pace. Ninhydrin, although the most versatile detection method, loses its effectiveness when quantitation below 100 picomoles of amino acid is required. Orthophthalaldehyde, although offering excellent sensitivity, suffers from poor quantitation of imino acids. The PTC chemi
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Manual Gas Phase Isothiocyanate Degradationships, molecular evolution, protein engineering, genetic engineering and post-translational modification. The automated gas-phase isothiocyanate degradation is a particularly well suited cyclic degradation procedure for such studies due to its sensitivity and high repetitive yield (Hewick et al 1981
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Accelerated High Sensitivity Sequence Analysis of Proteins and Peptides Immobilized on Chemically-mootein sequences could only be determined by combining extended N-terminal sequence information obtained from purified proteins with the sequences of overlapping cleavage fragments. Micromoles of purified protein and a tenacious protein chemist were needed to fill in the last gaps in the sequence. To
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