Titlebook: Methods in Protein Sequence Analysis; Proceedings of the 7 Brigitte Wittmann-Liebold Conference proceedings 1989 Springer-Verlag Berlin Hei

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Conference proceedings 1989he Seventh International Conference on "Methods in Protein Sequence Analysis", held from July 3rd to July 8th, 1988 in Berlin. The book contains information on new methodologies for sensitive amino acid analysis, N- and C-terminal sequence analysis, and protein and peptide purification. In addition

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Detection Limits in Amino Acid Analysis: An Overviewcial companies are promoting different approaches. What are the pros and cons of the different methods? What is the state-of-the-art? How serious is sample and reagent contamination or selective destruction during sample processing?

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Adulterate

CUB

Study on New Edman-type Reagentsl., 1986; Wittmann-Liebold et al., 1988). This reagent allowed sequence determination using 100 pmol and 1 nmol of insulin B chain for 9 and 19 cycles, respectively, with thin-layer chromatographic identification of the released fluorescent thiohydantoin amino acid derivatives (Hirano et al., 1986).

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豪华

PTCA635

PHIL

A Comparison of 9-Fluorenylmethylchloroformate (FMOC-Cl) Amino Acid Analysis with Ninhydrin and Phen of amino acid is required. Orthophthalaldehyde, although offering excellent sensitivity, suffers from poor quantitation of imino acids. The PTC chemistry offers a viable alternative but matrix effects associated with the sample can cause variable recoveries of glutamic and aspartic acids.

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Protein Microsequence Analysis with Dansylamino-PITC limitations for sequence analysis. For example, some of them carry a bulky side group which often sterically hinders the reactive group, resulting in a low coupling yield, and some produce a large number of reaction by-products caused by the side reactions. Such problems have restricted the use of these reagents in protein sequencing.