Titlebook: Metaproteomics; Methods and Protocol Carlo Salerno Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sp

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Proteomics for Microalgae Extracts by High-Resolution Mass Spectrometry,ased on an aqueous buffer solution of Tris–HCl and the second on cold acetone. The identification of proteins was carried out by a bottom-up approach, which involves enzymatic digestion of extracted proteins followed by either matrix-assisted laser desorption ionization with time-of-flight (MALDI-TO

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Metaproteomic Analysis of Microbial Communities Affecting Fishery Products,ed, during catching, technological processing, and storage. Detection and identification of pathogenic and spoilage microorganisms in fishery products is needed because the first may be involved in human diseases, while the second is responsible of significant economic losses. In this sense, liquid

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Metaproteomic Analysis of Fecal Samples from Human Subjects and Rodent Models,are involved in specific functions and pathways. This chapter describes a protocol enabling analysis and characterization of fecal metaproteomes, successfully applied to human, mouse, and rat stool samples. The protocol combines mechanical and thermal treatments for protein extraction, a centrifugal

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Fungal Secretomics Through iTRAQ-Based Analysis, Fungi produce a variety of secreted proteins, and environmental conditions can easily influence the fungal secretome. However, the low abundance of secreted proteins and their post-translational modifications make protein extraction more challenging. Hence, the enrichment of secreted proteins is a

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Bioinformatic Workflows for Metaproteomics,hniques for the investigation of the composition and function of microbial community samples have been developed. Metaproteomics, the comprehensive analysis of the proteins from microbial communities, allows for the investigation of not only the taxonomy but also the functional and quantitative comp