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Titlebook: Metaproteomics; Methods and Protocol Carlo Salerno Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sp

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Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics,d workflow including sampling, phenol extraction in a ball mill, amido black protein quantification, FASP digestion, LC-MS/MS measurement as well as bioinformatics and biostatistical data evaluation are here described for the metaproteomics advancements applied to biogas plant samples.
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Metaproteomic Analysis of Fecal Samples from Human Subjects and Rodent Models,essfully applied to human, mouse, and rat stool samples. The protocol combines mechanical and thermal treatments for protein extraction, a centrifugal filter-based procedure for cleanup and digestion, long-gradient liquid chromatography for peptide separation, and high-resolution mass spectrometry for peptide detection.
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Fungal Secretomics Through iTRAQ-Based Analysis,f fungi exposed to different environmental conditions. The fungal-secreted proteins can be extracted by combining ultrafiltration and TCA-acetone precipitation. Subsequently, the secreted proteins can be identified and quantified by the iTRAQ-based quantitative proteomics approach.
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