Titlebook: Memory B-Cells; Methods and Protocol Kim L. Good-Jacobson Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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Epigenomic Profiling of B Cell Subsets by CUT&Tag mechanisms, such as the post-translational modifications to histones, present several technical challenges that prevent a complete understanding of gene regulation. Here, we provide a detailed protocol of the Cleavage Under Targets and Tagmentation (CUT&Tag) chromatin profiling technique for identi

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Isolation of Rare Antigen-Specific Memory B Cells via Antigen Tetramerseeper understanding of the mechanisms underlying this phenomenon can aide in research aimed to improve vaccines and therapies. Memory B cells (MBCs) are a fundamental component of immunological memory but can exist in rare populations that prove challenging to study. By combining fluorescent antigen

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ELISpots for Detecting Antigen-Specific Memory B Cells in Infection or Autoimmunitycells cultured in ELISpot plates (96- or 8-well format) bind to a specific antigen bound to a PVDF membrane at the bottom of the well. A detection antibody followed by an enzymatic reaction is used to identify secreted protein bound to the membrane coated antigen. This reaction results in distinct “

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