Titlebook: Memory B-Cells; Methods and Protocol Kim L. Good-Jacobson Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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两种语言

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https://doi.org/10.1007/978-1-0716-3950-4Secondary pathogen exposure; Vaccine-induced immune memory; Antigen-specific memory B cells; Tetramers;

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In Vivo CRISPR/Cas9-Mediated Gene Ablation in Murine B Cellserentiation of germinal center B cells. Here, we describe an approach to perform an in vivo Crispr-Cas9 screen to specifically ablate genes in activated B cells. Using this approach, we have been able to reveal novel transcriptional regulators of germinal center B cell differentiation following viral infection.

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Using Ex Vivo Tonsil Organoids to Study Memory B Cellsvidual variation in response to a variety of stimuli. Lymphoid tissue organoids enable characterization of pre-existing antigen-specific MBCs within an entirely human system and can provide valuable insights into MBC dynamics.

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Flow Cytometric Identification of Human IgE+ B Lineage Subsetse used to robustly validate this approach before application to primary human samples. Orthogonal confirmatory techniques remain essential; these are not described in detail, but several possible strategies are suggested.