Titlebook: Memory B-Cells; Methods and Protocol Kim L. Good-Jacobson Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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Identification of IgG1 and IgG3 Allotypes by PCR and Sanger Sequencing(SNPs) responsible for the observed amino acid substitutions. Here, we provide a detailed protocol for the amplification of . and . segments by PCR, sample preparation for Sanger sequencing, and analysis of sequencing data to identify SNPs associated with different IgG1 and IgG3 allotypes.

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Visualizing Epigenetic Modifications and Nuclear Bodies by Immunofluorescence Staining in Naïve, Acte elucidation of their function. Here, we provide a protocol for immunofluorescence staining in the nucleus, which has successfully been used to visualize histone modifications and nuclear bodies in human and mouse B lymphocytes, using as few as 1 × 10.–5 × 10. cells.

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Measuring B Cell Chromatin Accessibility by One-Step ATAC-seqted and adapter tagged DNA is then purified and PCR amplified with dual indexing primers to generate a size-specific sequencing library. The One-Step workflow below outlines the Tn5 nuclei transposition from a range of cell inputs followed by PCR amplification to generate a sequencing library.

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Ultrasound-Guided Fine Needle Biopsy of Human Axillary Lymph Nodes to Assess B Cell Responses to Vacthe immune response within the lymph nodes following vaccination. Once acquired, lymph node cells can be characterized via flow cytometric immunophenotyping and/or single-cell RNA sequencing for gene expression and T and B cell receptors. Analysis of the immune cells from the lymph nodes enables the

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