Titlebook: Membrane Lipids; Methods and Protocol Charles G. Cranfield Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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Small-Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling,lution. As such, it is suitable to study preparations of homogeneous liposomes to extract simple structural parameters such as radius and lipid bilayer thickness in solution. Here, we describe the preparation of liposomes for small-angle neutron scattering via extrusion and present the simplest way

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Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation,intra- and inter-particle exchange often require monitoring the transfer of bulky and perturbing fluorescent labels. Time-resolved small angle neutron scattering (TR-SANS) overcomes these flaws by isotope labeling, allowing for the monomeric exchange rate determination of unperturbed, stress-free pa

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Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering,es. Using small angle neutron scattering, contrast-matching techniques enable the detection of lipid domains ranging from tens to hundreds of nanometers which are not accessible to other techniques without the use of extrinsic probes. Here, we describe a probe-free experimental approach and model-fr

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Using , to Model Neutron Reflectometry Data from Phospholipid Bilayers,lution (Penfold and Thomas, J Phys Condens Matter 2:1369–1412, 1990). This type of experiment is undertaken at large international central facilities and experience in data analysis and interpretation is not always available “locally”. Here, we describe the application of the . software suite (Nelso

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Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating B hydration of lipid polar heads in a proximal leaflet and the possibility to generate the potential gradient across the membrane. This chapter describes the protocol for the measurement of interfacial water separating the floating bilayer lipid membrane from the solid support using surface-enhanced

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Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Likst. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated li

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