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Titlebook: Membrane Lipids; Methods and Protocol Charles G. Cranfield Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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书目名称Membrane Lipids
副标题Methods and Protocol
编辑Charles G. Cranfield
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Membrane Lipids; Methods and Protocol Charles G. Cranfield Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen
描述This detailed book explores examples of current .in vitro. and .in silico. techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer .in silico. molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful .Methods in Molecular Biology. series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and cutting-edge, .Membrane Lipids: Methods and Protocols. serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics..
出版日期Book 2022
关键词Lipid bilayers; Membrane-bound ion channels; Biophysical dynamics; Electrical impedance spectroscopy; In
版次1
doihttps://doi.org/10.1007/978-1-0716-1843-1
isbn_softcover978-1-0716-1845-5
isbn_ebook978-1-0716-1843-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/630365.jpg
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Methods for Forming Giant Unilamellar Fatty Acid Vesicles, of origins of life, soft matter science, biophysics, and drug delivery, but are also cost-effective and easy to manipulate, making them ideal for teaching students about self-assembly and lipid bilayers. Here, we describe simple ways to make giant, unilamellar fatty acid vesicles suitable for microscopy and encapsulation studies.
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Preparing Ion Channel Switch Membrane-Based Biosensors, a specific target molecule. These biosensors represent the amalgamation of an electrical conductor such as gold and a chemically tethered bilayer lipid membrane with specific incorporated ion channels such as gramicidin-A that is further functionalized with detector molecules of interest.
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,Measuring Voltage–Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the ElecTethered bilayer lipid membranes (tBLMs) anchored to a solid substrate can be prepared and individual triangular voltage ramps from zero to 500 mV with a period of 2–10 ms applied to give membrane voltage dependencies with and without the addition of drugs and analytes in order to measure their electro-insertion properties.
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