Titlebook: Meiosis; Volume 1, Molecular Scott Keeney Book 2009 Humana Press 2009 Arabidopsis thaliana.Budding and fission yeasts.Chromosome dynamics.

compassion

集聚成团

CHOKE

ALIBI

Measurement of Spatial Proximity and Accessibility of Chromosomal Loci in , Using Cre /, Site-Specifanalysis of fixed, spread nuclei using fluorescence in situ hybridization (FISH) .1, 2., visualization of GFP-labeled chromosomal loci in living cells .3., and Chromosome-Conformation Capture (3C) .4.. Here we describe a quantitative genetic assay that uses exogenous site-specific recombination to m

Culmination

Analysis of Meiotic Recombination in , of meiotic recombination in ., ranging from traditional genetic measures to direct cytological determination of chiasma frequency. Here, we provide methods for some of the varied approaches used for the study of meiotic recombination in these tiny but powerful worms.

Protein

GLUE

Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in , at Various Spatial Resolution manner. Meiotic DSBs can be detected directly using physical assays (gel electrophoresis, Southern blotting, and indirect end-labeling) applied to samples of genomic DNA from sporulating cultures of budding and fission yeast. Such assays are extremely useful for quantifying and characterizing many

GNAW

Admonish

Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cellsrogression through meiosis is not synchronous and only a small fraction of all testis cells are actually at the stage where DSB formation is initiated. We devised a quantitative approach that is sensitive enough to detect the position of rare DNA strand breaks in mouse germ cell-enriched testicular

B-cell

End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in ,g this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extrac