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Titlebook: Meiosis; Volume 1, Molecular Scott Keeney Book 2009 Humana Press 2009 Arabidopsis thaliana.Budding and fission yeasts.Chromosome dynamics.

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书目名称Meiosis
副标题Volume 1, Molecular
编辑Scott Keeney
视频video
概述State-of-the-art techniques for analysis of meiotic recombination.Comprehensive methods for studying meiosis in human and in many commonly used model organisms.Chapters on background theory, methodolo
丛书名称Methods in Molecular Biology
图书封面Titlebook: Meiosis; Volume 1, Molecular  Scott Keeney Book 2009 Humana Press 2009 Arabidopsis thaliana.Budding and fission yeasts.Chromosome dynamics.
描述Each generation in a sexually reproducing organism such as a fly or a mouse passes through the bottleneck of meiosis, which is the specialized cell division that gives rise to haploid reproductive cells (sperm, eggs, spores, etc. ). The principal function of meiosis is to reduce the genome complement by half, which is accomplished through sequential execution of one round of DNA replication followed by two rounds of chromosome segregation. Within the extended prophase between DNA replication and the first meiotic division in most organisms, homologous maternal and paternal chromosomes pair with one another and undergo homologous recombination, which establishes physical connections that link the homologous chromosomes until the time they are separated at anaphase I. Recombination also serves to increase genetic diversity from one generation to the next by breaking up linkage groups. The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been an explosion of new information about how meiotic chromosomes pair, recombine, and are segregated. Progress has been driven by advances in three main areas: (1) genetic identi
出版日期Book 2009
关键词Arabidopsis thaliana; Budding and fission yeasts; Chromosome dynamics; DNA; Meiotic recombination; Protei
版次1
doihttps://doi.org/10.1007/978-1-59745-527-5
isbn_softcover978-1-4939-6157-3
isbn_ebook978-1-59745-527-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

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Analysis of Meiotic Recombination in , of meiotic recombination in ., ranging from traditional genetic measures to direct cytological determination of chiasma frequency. Here, we provide methods for some of the varied approaches used for the study of meiotic recombination in these tiny but powerful worms.
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End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in ,g this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells.
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Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/630258.jpg
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