Titlebook: Meiosis; David T. Stuart Book 2017Latest edition Springer Science+Business Media New York 2017 Gametogenesis.Budding yeast models.Computat

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David T. StuartIncludes cutting-edge methods and protocols for meiosis research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes

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Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/630256.jpg

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, Male Meiosis,ate chromosome segregation. In addition, due to their large size, the meiotic spindles of . males are an excellent system for mutational dissection of the mechanisms of spindle assembly. Here, we describe the main techniques for visualization of live . testes and for preparation of fixed meiotic chromosomes and spindles.

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978-1-4939-8169-4Springer Science+Business Media New York 2017

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Meiosis978-1-4939-6340-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein Binding in ,,observed, creating a better understanding of how recombination is regulated. Additional genome-wide analyses have shown proteins that bind specifically to DSB hotspots, providing insight into how the DSB initiation complex functions. We describe here detailed methods for achieving these results.

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Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules Durisuch as recombination nodules, can be identified by EM regardless of whether they are labeled or not. However, immunogold localization has other disadvantages including comparatively low signal-to-noise ratios, more difficult colocalization using gold particles of different sizes, and the inability