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Titlebook: Meiosis; David T. Stuart Book 2017Latest edition Springer Science+Business Media New York 2017 Gametogenesis.Budding yeast models.Computat

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Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules Durioint proteins involved in recombination during meiotic prophase. Each method has its advantages and disadvantages. For example, LM immunofluorescence is comparatively easier and higher throughput compared to immunogold EM localization. In addition, immunofluorescence has the advantages that a faint
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Flow Cytometry for the Isolation and Characterization of Rodent Meiocytes,y true for early meiotic prophase stages (leptotene and zygotene). Here, we describe a method for obtaining cells in different spermatogenic stages from rodents including lepto-zygotene meiocytes at very high purity levels. The procedure includes an approach for the mechanical disaggregation of the
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Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH,tant insights into chromosome dynamics during the complex meiotic differentiation program that compensates for the genome doubling at fertilization. Homologous chromosomes (homologues) undergo dynamic pairing and recombine during first meiotic prophase before they become partitioned into four haploi
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Live Imaging of Meiosis I in Late-Stage , Oocytes,it has only been about 25 years since the events that occur during nuclear envelope breakdown, spindle assembly, and chromosome orientation during . female meiosis I were first visualized by fixed cytological methods (Theurkauf and Hawley, J Cell Biol 116:1167–1180, 1992). Although these fixed cytol
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Analysis of Chromatin Dynamics During , Spermatogenesis,sible to study the process of spermatogenesis in its entirety without detailed morphological analyses. Here, we describe a method to visualize chromatin dynamics in differentiating . male germ cells using immunofluorescence staining. In addition, we demonstrate how to treat . sperm before immunofluo
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