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Titlebook: Meiosis; David T. Stuart Book 2017Latest edition Springer Science+Business Media New York 2017 Gametogenesis.Budding yeast models.Computat

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发表于 2025-3-21 19:55:01 | 显示全部楼层 |阅读模式
书目名称Meiosis
编辑David T. Stuart
视频video
概述Includes cutting-edge methods and protocols for meiosis research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes
丛书名称Methods in Molecular Biology
图书封面Titlebook: Meiosis;  David T. Stuart Book 2017Latest edition Springer Science+Business Media New York 2017 Gametogenesis.Budding yeast models.Computat
描述This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Detailedand practical, .Meiosis, Second Edition. will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation..
出版日期Book 2017Latest edition
关键词Gametogenesis; Budding yeast models; Computational methods; Chromosome reorganization; Gene expression n
版次2
doihttps://doi.org/10.1007/978-1-4939-6340-9
isbn_softcover978-1-4939-8169-4
isbn_ebook978-1-4939-6340-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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发表于 2025-3-21 23:36:04 | 显示全部楼层
Book 2017Latest editionl methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies
发表于 2025-3-22 03:29:02 | 显示全部楼层
Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in ,,nation and chromosome segregation. Here we describe genetic methods for analysis progression of . through meiosis and sporulation with an emphasis on strategies for the genetic analysis of regulators of meiosis-specific genes.
发表于 2025-3-22 08:02:23 | 显示全部楼层
Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy,ytes in detail as they undergo maturation. Our method is based on tracking the “center of brightness” of fluorescently labeled chromosomes. Here we describe how to set up our software and run experiments on a Leica TCS SP8 confocal microscope, but the method would be transferable to other microscopes with computer-aided microscopy.
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Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast,tions of DNA breaks to the base pair. In this chapter we detail the steps involved in Spo11-oligo mapping in the SK1 strain of budding yeast ., from harvesting cells of highly synchronous meiotic cultures, through preparation of sequencing libraries, to the mapping pipeline used for processing the data.
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Fluorescent Protein as a Tool for Investigating Meiotic Recombination in ,,nalysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for .. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured.
发表于 2025-3-23 05:45:10 | 显示全部楼层
In Vivo Imaging of Budding Yeast Meiosis,is method allows tracking of individual cells over extended periods of time through every stage of the meiotic transformation while minimizing phototoxicity and sustaining conditions that support meiotic growth.
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