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Titlebook: Mass Spectrometry-Based Proteomics; Kris Gevaert Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr

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Automated Sample Preparation for Mass Spectrometry-Based Clinical Proteomics,roteolysis. It enables the concurrent processing of 96 clinical samples of any type (fresh-frozen or FFPE tissue, liquid biopsies, or cells) on an automated liquid handling platform, which can be directly interfaced to LC–MS for proteome analysis of clinical specimens with high sensitivity, high reproducibility, and short turn-around times.
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Phosphoproteomics After Guanidinium Thiocyanate Extraction of Tissue Biopsies,phenol-chloroform (AGPC) extraction with RNA-Bee. We describe the necessary steps for biopsy collection, cryoprocessing, and protein extraction. We further share our practice on protein digestion and cleanup of small samples (200 μg protein) and describe settings for automated IMAC-based phosphopeptide enrichment with the AssayMAP Bravo platform.
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Jade I. Hawksworth,Lode Denolf,Evy Timmerman,Kris Gevaert
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Esperanza Fernández,Laia Miret-Casals,Annemieke Madder,Kris Gevaert
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Reuben Samson,Francesco Zangari,Anne-Claude Gingras
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George D. Moschonas,Margaux De Meyer,Delphine De Sutter,Evy Timmerman,Petra Van Damme,Sven Eyckerman
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