书目名称 | Mass Spectrometry-Based Proteomics | 编辑 | Kris Gevaert | 视频video | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters on automated preparation of clinical samples, the analysis of formalin-fixed paraffin-embedded samples, protocols for the isolation of extracellular vesicles and for the monitoring of selected protein modifications in clinical samples, and, finally, structural proteomics. Written for the highly successful .Methods in Molecular Biology .series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Mass Spectrometry-Based Proteomics. serves as an ideal guide to its subject for both novices in the field o | 出版日期 | Book 2023 | 关键词 | Proteases; Proteome profiling; Clinical proteomics; Protein modifications; Cellular surfaceome | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-3457-8 | isbn_softcover | 978-1-0716-3459-2 | isbn_ebook | 978-1-0716-3457-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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