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Titlebook: Mass Spectrometry-Based Proteomics; Kris Gevaert Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr

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发表于 2025-3-21 19:31:08 | 显示全部楼层 |阅读模式
书目名称Mass Spectrometry-Based Proteomics
编辑Kris Gevaert
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Mass Spectrometry-Based Proteomics;  Kris Gevaert Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr
描述This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters on automated preparation of clinical samples, the analysis of formalin-fixed paraffin-embedded samples, protocols for the isolation of extracellular vesicles and for the monitoring of selected protein modifications in clinical samples, and, finally, structural proteomics. Written for the highly successful .Methods in Molecular Biology .series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Mass Spectrometry-Based Proteomics. serves as an ideal guide to its subject for both novices in the field o
出版日期Book 2023
关键词Proteases; Proteome profiling; Clinical proteomics; Protein modifications; Cellular surfaceome
版次1
doihttps://doi.org/10.1007/978-1-0716-3457-8
isbn_softcover978-1-0716-3459-2
isbn_ebook978-1-0716-3457-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Sensitive and High-Throughput Exploration of Protein N-Termini by TMT-TAILS N-Terminomics,vestigation of differential proteolysis, protease substrate discovery, and analysis of N-terminal PTMs. In this chapter, we provide a state-of-the-art protocol, with detailed steps in all parts of the TAILS sample preparation, MS analysis, and post-processing of acquired data.
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Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry,pter, we describe a universal HDX-MS workflow. Deeper attention is paid to sample handling, optimization procedures, and feasibility stages, as these elements of the HDX experiment are crucial for obtaining reliable detailed and spatially well-resolved information.
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Book 2023 surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosph
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Density-Based Fractionation of Cell-Conditioned Medium to Prepare Proteomics Grade Extracellular Vecentrifugation and size-exclusion chromatography to prepare EV from cell-conditioned medium with high specificity and repeatability. This approach enables the recovery of intact purified EV suited for downstream functional assays and biomarker discovery by omics approaches.
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Thermal Proteome Profiling for Drug Target Identification and Probing of Protein States, experiments using live cells or crude cell extracts. We provide the tools to perform different TPP formats, i.e., temperature range experiments, TPP-TR; isothermal compound titrations, TPP-CCR; and a combination thereof, 2D-TPP.
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