Titlebook: Mass Spectrometry of Proteins; Methods and Protocol Caroline A.‘Evans,Phillip C. Wright,Josselin Noire Book 2019 Springer Science+Business

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Dana Pascovici,Xiaomin Song,Jemma Wu,Thiri Zaw,Mark Molloy

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Andrew F. Jarnuczak,Tobias Ternent,Juan Antonio Vizcaíno

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Assessment of Ubiquitin Chain Topology by Targeted Mass Spectrometrytin chains in the sample have to be biochemically stabilized. Second, the selection of peptides for the analysis is restricted to a given set harboring the PTMs and does not allow for optimization for amenability to mass spectrometry-based quantification. Instead, the topology-characteristic peptide

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Considerations for Identifying Endogenous Protein Complexes from Tissue via Immunoaffinity Purificatmple, the limited use of this approach has been primarily driven by challenges associated with finding adequate antibodies and experimental conditions for effective isolations. In this chapter, we present a protocol for the optimization of lysis conditions, antibody evaluation, affinity purification