书目名称 | Mass Spectrometry of Proteins | 副标题 | Methods and Protocol | 编辑 | Caroline A.‘Evans,Phillip C. Wright,Josselin Noire | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With applications to biomarker discovery, interactions between proteins, between biological systems, dynamics of post-translational modifications among others, new protocols have been developed and iteratively refined to probe the endless complexity of the proteome in ever greater details. This volume deals with methods for data dependent and data independent mass spectrometry analyses. Valuable, first-hand information is provided from designing experiments, sample preparation and analysis, exploitation of public datasets and carrying out reproducible data pipelines, using modern computational tools such as Galaxy or Jupyter. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and a | 出版日期 | Book 2019 | 关键词 | iTRAQ; TMT; di methyl labelling; QConCat; quantitative proteomics | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-9232-4 | isbn_softcover | 978-1-4939-9234-8 | isbn_ebook | 978-1-4939-9232-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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