Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Arnaud Delobel Book 2021 Springer Science+Business Media, LLC, part of Springer Na

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Fc Glycosylation Characterization of Human Immunoglobulins G Using Immunocapture and LC-MS,nsitive and reproducible analytical approach..This chapter describes an optimized approach for the preparation and the analysis of Fc .-glycans from total serum or plasma IgG using magnetic beads, RapiFluor MS label©, and LC-MS.

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Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophiction chromatography (SAX-ERLIC). Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis. Glycopeptide data generated using the SAX-ERLIC enrichment yields a high numb