Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Arnaud Delobel Book 2021 Springer Science+Business Media, LLC, part of Springer Na

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Use of Exoglycosidases for the Structural Characterization of Glycans,ct labeling of released glycans with procainamide (PCA) or 2-aminobenzamide (2AB), cleanup of labeled glycans and a 3 h enzymatic digestion with exoglycosidases. This protocol is designed for completion within an 8 h time frame to allow for subsequent LC-FLD, LC-MS, or CE analysis overnight.

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Book 2021sses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact p

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1064-3745 ation advice from the expertsThis volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in thi

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Mapping ,-glycosylation Sites Using OpeRATOR and LC-MS,s enables the generation of glycopeptides of suitable size for mapping .-glycosylation sites in detail by bottom-up LC-MS analysis. In this chapter we demonstrate a simple workflow for in-depth analysis of .-glycosylation sites on heavily glycosylated proteins using OpeRATOR digestion and HILIC-MS/MS analysis.

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Profiling, Relative Quantification, and Identification of Sialylated ,-Linked Oligosaccharides by Uarge and the sialylation level is determined based on the fluorescence signal. In addition, in this method, the .-glycans are also separated by type and identified with the hyphenated MS. For both methods, an optimized protocol with fast and high-throughput sample preparation and purification is presented.

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1064-3745 known pitfalls..Cutting-edge and thorough, .Mass Spectrometry of Glycoproteins: Methods and Protocols. is a valuable resource for scientists interested in learning more about this978-1-0716-1243-9978-1-0716-1241-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Yosra Helali,Shilpee Sharma,Marie Vandeput,Dansala Welba,Pierre Van Antwerpen,Arnaud Marchant,Cédric