Titlebook: Mammalian Synthetic Systems; Francesca Ceroni,Karen Polizzi Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive lic

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Setup and Applications of Modular Protein Expression Toolboxes (MoPET) for Mammalian Systems, modules effecting protein expression with a focus on minimizing the cloning burden in coding regions. The original MoPET system consists of 53 defined DNA modules divided into eight functional main classes and can be flexibly expanded dependent on the need of the experimenter and expression host. H

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Anti-CRISPR Proteins and Their Application to Control CRISPR Effectors in Mammalian Systems,controlling CRISPR effectors in heterologous systems, including mammalian cells..Here, we review the diverse applications of Acrs in mammalian cells and organisms and discuss the underlying engineering strategies. These applications include (i) persistent blockage of CRISPR-Cas function to create wr

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