Titlebook: Mammalian Synthetic Systems; Francesca Ceroni,Karen Polizzi Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive lic

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High-Throughput Spectroscopic Analysis of mRNA Capping Level,e sensitivity to increases in capping level of at least 20% and a limit of detection of 2.4 pmol of mRNA. Overall, the biosensor can provide key information about mRNA quality before mammalian cell transfection.

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OTTER

RNA Switches Using Cas Proteins,nd the available tools in synthetic biology because of the wide range of Cas protein orthologs that can be used as trigger proteins..Here, we describe the design principle of Cas protein-responsive switches, both plasmid and RNA versions, using . Cas9 (SpCas9) as an example and show an example of its use in mammalian cells, HEK293FT cells.

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Ovulation

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Engineered Protease-Responsive RNA-Binding Proteins (RBPs) to Expand the Toolbox of Synthetic Circue sophisticated and multilayered regulatory networks, addressing the previous limitation of regulatory orthogonal parts that can be assembled in synthetic devices. In this chapter, we describe the engineering and tests of protease-responsive RNA-binding proteins (L7Ae and MS2-cNOT7) to expand the toolbox of synthetic circuits in mammalian cells.

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Repatriate

Detection of MicroRNAs Using Synthetic Toehold Switch in Mammalian Cells,tic toehold switch for detection of exogenously and endogenously expressed miRNAs in CHO, HeLa, HEK 293, and MDA-MB-231 breast cancer cells. Multiplex detection of miR-155 and miR-21 was tested using two toehold switches to evaluate the orthogonality and programmability of this synthetic platform.

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