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Titlebook: Mammalian Synthetic Systems; Francesca Ceroni,Karen Polizzi Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive lic

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书目名称Mammalian Synthetic Systems
编辑Francesca Ceroni,Karen Polizzi
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Mammalian Synthetic Systems;  Francesca Ceroni,Karen Polizzi Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive lic
描述.This volume details cutting-edge methods that enables engineering of novel functions in mammalian cells. Chapters guide readers through cell-to-cell communication, cell fate control, protein and RNA-based biosensors together with tools for more reliable and faster mammalian genome editing. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.. ..Authoritative and cutting-edge, .Mammalian Synthetic Systems .aims to ensure successful results in the further study of this vital field..
出版日期Book 2024
关键词Cloning toolkits; Gene expression control; Synthetic epigenetics; CRISPR-based systems; Bioprocessing ap
版次1
doihttps://doi.org/10.1007/978-1-0716-3718-0
isbn_softcover978-1-0716-3720-3
isbn_ebook978-1-0716-3718-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Setup and Applications of Modular Protein Expression Toolboxes (MoPET) for Mammalian Systems,t. However, the exchange and modification of the coding and/or noncoding elements to analyze their effect on protein function or generating the optimal result can be a tedious and time-consuming process using standard molecular biology cloning methods. To streamline the process to generate defined e
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,A Mammalian-Based Synthetic Biology Toolbox to Engineer Membrane–Membrane Interfaces,s cellular processes including cell signaling, development, and immunity. Using synthetic biology to engineer or to create novel cellular functions in the intercellular regions has led to an increasing need for a platform that allows generation of functionalized intercellular membrane–membrane inter
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Multiplex Functional Characterization of Protein Variant Libraries in Mammalian Cells with Single-Cncoding thousands of unique protein variants. Since plasmid transfection and lentiviral transduction have characteristics that limit multiplexing with pooled libraries, we developed a mammalian synthetic biology platform that harnesses the Bxb1 bacteriophage DNA recombinase to insert single promoter
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