Titlebook: Malaria Immunology; Targeting the Surfac Anja Tatiana Ramstedt Jensen,Lars Hviid Book 2022 The Editor(s) (if applicable) and The Author(s),

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Synchronisation of , and , In Vitro Cultures Using a Highly Specific Protein Kinase Inhibitorased merozoites. Additionally, we describe how to use ML10 to improve the transfection efficiency of . parasites and also how to derive the half maximal effective concentration (EC.) of ML10 in other . laboratory lines and clinical isolates.

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Analysis of var Gene Transcription Pattern Using DBLα Tagsenes are highly variable, which makes it difficult to study their expression in clinical isolates obtained directly from malaria patients. In this chapter, we describe an approach for analysis of . gene expression that targets a region referred to as DBLα tag, which is relatively conserved in all . genes.

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Preservation and Extraction of Malaria Parasite DNA from Dried Blood Spotsn have been described, and many comparative studies have analyzed and optimized the different methodologies to find an alternative to the more costly commercial extraction kits. This chapter describes recommendations for storage and preservation of DBS, and a Chelex-based protocol for extraction of

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1064-3745 ther their study in this field. .Chapter Analysis of var gene transcription pattern using DBLα-tags [Chapter 14] is available open access under a Creative Commons Attribution 4.0  International License via link.springer.com..978-1-0716-2191-2978-1-0716-2189-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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