Titlebook: Malaria Immunology; Targeting the Surfac Anja Tatiana Ramstedt Jensen,Lars Hviid Book 2022 The Editor(s) (if applicable) and The Author(s),

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1064-3745 ation advice from the experts.This volume covers a broad range of methods, technologies, and protocols on malaria. Chapters detail research on collecting parasites in the field, single molecule-level analyses of adhesive interactions, and focused studies aiming at disrupting the devastating disease.

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Book 2022nology: Targeting the Surface of Infected Erythrocytes .aims to be a useful and practical guide to researches to help further their study in this field. .Chapter Analysis of var gene transcription pattern using DBLα-tags [Chapter 14] is available open access under a Creative Commons Attribution 4.0  International License via link.springer.com..

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Collection and Cryopreservation of , Clinical Isolates in the Fieldres technical expertise and equipment. To overcome this challenge, clinical isolates are cryopreserved in the field and transported to a laboratory for in vitro studies. In this protocol, we describe different methods of cryopreserving . isolates in the field and thawing them for subsequent in vitro culture.

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Preservation of Parasite RNA in the Fieldute prerequisite for a precise and accurate transcriptome profiling. Immediate stabilization of RNA of biological samples is therefore necessary to prevent degradation by ribonucleases (RNase) or cellular changes. This chapter described methodology for preserving parasite RNA samples from malaria patients in the field for transcriptome studies.

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Selecting , Infected Erythrocytes for Adhesion to Cell LinesEs on cell lines. The method enables coculturing of IEs with cells of interest and the selection of IEs that cytoadhere for continuous culturing. The method serves as a tool for generating IEs with specific cell or cell receptor adhesion phenotypes to allow detailed studies of cytoadhesion interactions.

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