Titlebook: Malaria Immunology; Targeting the Surfac Anja Tatiana Ramstedt Jensen,Lars Hviid Book 2022 The Editor(s) (if applicable) and The Author(s),

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Resetting , Gene Transcription in e PfEMP1 adhesive proteins, encoded by the . gene family. To avoid premature exposure of its antigenic repertoire, the parasite transcribes its . genes in a mutually exclusive manner, and switch expression at a very slow rate. This process is epigenetically regulated and it relies on “epigenetic mem

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978-1-0716-2191-2The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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anachronistic

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Immunomagnetic Selection of ,–Infected Erythrocytes Expressing Particular PfEMP1 Variants family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.

Nmda-Receptor

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CRISPR/Cas9 Editing of the , Genometions ranges from single point mutations to large multigene deletions, however many of the technical considerations for designing CRISPR-based experiments are common to any editing approach. This review will discuss protocols for vector construction and donor design for genome editing ., including pitfalls, variables, and validation methods.

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Anja Tatiana Ramstedt Jensen,Lars HviidIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts