Titlebook: Macrophages; Methods and Protocol Germain Rousselet Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 phenotypin

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书目名称Macrophages影响因子(影响力)




书目名称Macrophages影响因子(影响力)学科排名




书目名称Macrophages网络公开度




书目名称Macrophages网络公开度学科排名




书目名称Macrophages被引频次




书目名称Macrophages被引频次学科排名




书目名称Macrophages年度引用




书目名称Macrophages年度引用学科排名




书目名称Macrophages读者反馈




书目名称Macrophages读者反馈学科排名

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1064-3745 ation advice from the expertsThis volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues;

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Isolation and Phenotyping of Bone Marrow Macrophages,ges are part of the hematopoietic stem cell niche..Those macrophages are known to have a role in the support of erythropoiesis (Chow, Nat Med 19:429–436, 2013), the maintenance of stem cell in their niches (Chow, J Exp Med 208:261–271, 2011), and are an independent self-renewing population (Hashimoto, Immunity 38:792–804, 2013).

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Isolation and Phenotyping of Intestinal Macrophages,hages are a heterogeneous population of immune cells that fulfill tissue-specific and niche-specific functions. These unique phenotypes likely reflect the heterogeneity of tissue macrophage origins and influence the tissue environment in which they reside. Here we describe how we can characterize and isolate the colonic macrophages.

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Activating Murine Macrophages In Vitro,tion in vitro, as these cells are relatively hardy and many macrophages can be produced from just one mouse. Here we describe the activation of mouse macrophages in vitro, including special considerations for cell culture, detachment, kinetics, and activation states.

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A Simple Multistep Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functionao functional macrophages. This includes derivation of three germ-line containing embryoid bodies (EBs) from iPSCs, generation of myeloid precursors from EBs, and finally maturation of myeloid precursors into functional macrophages. Technical procedure and specific culture conditions associated with each of these steps are discussed in detail.

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Viral Replication Assay in Bone Marrow-Derived Macrophages,lls contribute significantly to MCMV biology and, thus, are actively recruited by the virus-encoded chemokine. In this chapter, we provide detailed methodologies employed in our laboratory to assess MCMV replication in bone marrow-derived macrophages.

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