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Titlebook: Macrophages; Methods and Protocol Germain Rousselet Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 phenotypin

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Quantitative Phagocytosis Assays in Primary and Cultured Macrophages,includes methods for preparation of phagocytic targets, specifically sheep erythrocytes opsonized with immunoglobulin G (IgG), as well as means of distinguishing bound from internalized targets, using fluorescently labeled secondary antibodies.
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In Vitro Migration Assays,chamber, first described in 1962. In this chapter we give detailed protocols for more recent techniques that allow determination of macrophage chemotaxis in real time. These techniques have given new insights into the regulation of macrophage responses to chemotaxis in vitro and in vivo.
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Chandrayana Mukherjee,Christine Hale,Subhankar Mukhopadhyay
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Anaïs Assouvie,Lisa P. Daley-Bauer,Germain Rousselet
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Shaikh M. Atif,Sophie L. Gibbings,Claudia V. Jakubzick
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