Titlebook: MAIT Cells; Methods and Protocol Helen Kaipe,Isabelle Magalhaes Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 202

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书目名称MAIT Cells影响因子(影响力)




书目名称MAIT Cells影响因子(影响力)学科排名




书目名称MAIT Cells网络公开度




书目名称MAIT Cells网络公开度学科排名




书目名称MAIT Cells被引频次




书目名称MAIT Cells被引频次学科排名




书目名称MAIT Cells年度引用




书目名称MAIT Cells年度引用学科排名




书目名称MAIT Cells读者反馈




书目名称MAIT Cells读者反馈学科排名

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1064-3745 ation advice from the experts.This volume focuses on various methods used by researchers to study mucosal-associated invariant T (MAIT) cells in the aspects described below. The chapters are organized into Four Parts: part One looks at the function and importance of MAIT cells in health and disease.

抛射物

MAIT Cells in Health and Diseaseve provided more insight into the development, mode of activation, characterization in different diseases and tissues of MAIT cells. In this chapter, we provide an overview of MAIT cells and yet remaining questions about their potential therapeutic role.

继而发生

Influenza A Virus-Infected Lung Epithelial Cell Co-Culture with Human Peripheral Blood Mononuclear Cytes in co-culture with an IAV-infected human lung epithelial cell line (A549) to measure ex vivo effector functions (TNF and IFNγ) in a mixed culture environment. We describe (1) infection of the human lung epithelial cell line, (2) co-culture with PBMC, and (3) measurement of activation using intracellular cytokine staining.

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A Flow Chamber Assay for Studying MAIT Cell Traffickingctivated cell sorting, we have used flow chambers to demonstrate roles for individual chemokine receptors in specific steps required for extravasation. These methods provide a general way to study the molecular mechanisms underlying MAIT cell trafficking from blood into tissue.

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厚颜

In Situ Detection of MAIT Cells and MR1-Expressing Cells in Tissue Biopsies Utilizing Immunohistocheues to detect MAIT cells and MR1-expressing cells in situ, which enable the visualization, distribution, and localization of these cells within their histological context. We provide specific protocols and describe potential advantages and limitations for each of the presented methodologies for studying MAIT cells in human tissues.

Kidnap

Immune Monitoring of Human Mucosal-Associated Invariant T Cells by Quantitative Proteomicsblood donations by FACS and are then characterized by quantitative proteomics (iTRAQ-LC-MS/MS) to complement information about their unique effector phenotype and to investigate donor-/patient- or disease-specific variations in protein networks with high precision.

Ornithologist

Isolation and Characterization of MAIT Cells from Tumor Tissuesromoting protective tumor immunity. Here, we describe enzymatic methods to isolate intraepithelial and lamina propria lymphocyte single cell suspensions from colon tissue and tumors containing viable MAIT cells, which can be used for further purification, flow cytometry analysis, or culture.