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Titlebook: Low-Temperature Microscopy and Analysis; Patrick Echlin Book 1992 Springer Science+Business Media New York 1992 Hydra.biological.electron.

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Low-Temperature Light Microscopy,roscope, . observer were all in a cold environment. Cohn (1871) and Kunisch (1880) went outdoors in winter to study the process of freezing in cells of the alga .. Weigand (1906) did the same thing to study freezing in fruit tree buds. The German botanist Sachs (1892) was a little more cautious and
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Low-Temperature Transmission Electron Microscopy,n. The images of most organic and biological material examined in the TEM have a very low amplitude contrast, which can only be ameliorated by staining with heavy metals. The presence of water in almost all biological material was solved by the draconian expedient of removing it and then replacing i
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serve dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro­ molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all t978-1-4899-2304-2978-1-4899-2302-8
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The Properties and Structure of Water, either as ice or amorphous water, is an important structural component. For this reason, water merits special attention and it is necessary to appreciate some basic facts about the compound, first at normal temperatures and pressures, and then at low temperatures.
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