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Titlebook: Low-Temperature Microscopy and Analysis; Patrick Echlin Book 1992 Springer Science+Business Media New York 1992 Hydra.biological.electron.

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书目名称Low-Temperature Microscopy and Analysis
编辑Patrick Echlin
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图书封面Titlebook: Low-Temperature Microscopy and Analysis;  Patrick Echlin Book 1992 Springer Science+Business Media New York 1992 Hydra.biological.electron.
描述The frozen-hydrated specimen is the principal element that unifies the subject of low­ temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com­ mon fact and theory upon which their work must be based. Low-Temperature Micros­ copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro­ molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all t
出版日期Book 1992
关键词Hydra; biological; electron; electron microscopy; illustration; low temperature; microscopy; replication; st
版次1
doihttps://doi.org/10.1007/978-1-4899-2302-8
isbn_softcover978-1-4899-2304-2
isbn_ebook978-1-4899-2302-8
copyrightSpringer Science+Business Media New York 1992
The information of publication is updating

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Low-Temperature Fracturing and Freeze-Fracture Replication,es of sections. There are fewer surface defects and artifacts on a fracture face of a frozen sample, provided the fracturing process is carried out in conditions of low contamination, such as under liquid nitrogen and at a high vacuum and low temperature.
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are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure an
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