Titlebook: Liver Carcinogenesis; Methods and Protocol Guido Kroemer,Jonathan Pol,Isabelle Martins Book 2024 The Editor(s) (if applicable) and The Auth

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A Mouse Model of Non-Alcoholic Steatohepatitis and Hepatocellular Carcinoma Induced by Western Dietturn, increases the risk of developing cirrhosis (liver scarring) and hepatocellular carcinoma (HCC). In addition to being a potentially life-threatening condition, public health concerns surrounding NASH are amplified by the lack of FDA-approved treatments. Although various preclinical models refle

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Hydrodynamic Transfection of Hepatocytes for the Study of Hepatocellular Carcinogenesis,enesis. This highly versatile approach reproducibly provides efficient in vivo transfection of hepatocytes with naked DNA. Here, we give an in-depth description of the injection procedure, which is key for the success of the method. HTVi requires the injection of a large volume of a solution contain

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Immunofluorescent Staining of Human Hepatic Multicellular Spheroids: A Model for Studying Liver Distwo-dimensional (2D) cultures of isolated primary cells or immortalized cell lines. However, this approach has limitations, as 2D cultures inadequately replicate the cell–cell and cell–extracellular matrix interactions found in three-dimensional (3D) environments. To overcome this limitation, variou

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