Titlebook: Liver Carcinogenesis; Methods and Protocol Guido Kroemer,Jonathan Pol,Isabelle Martins Book 2024 The Editor(s) (if applicable) and The Auth

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Chromatin and DNA Dynamics in Mouse Models of Liver Cancers,ty, histone mark deposition, as well as with chromatin looping establishing connectivity with regulatory regions. The emergence of new techniques for genome-wide analyses and their progressive optimization to work on low amounts of material allows the scientific community to obtain an integrated vie

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Targeted Analysis of Glycerophospholipids and Mono-, Di-, or Tri-Acylglycerides in Liver Cancer,y novel and more efficient diagnostic biomarkers and therapeutic targets based on the metabolic mechanisms of these diseases. In comparison to traditional diagnostic biomarkers, metabolomics is a comprehensive technique for discovering chemical signatures for liver cancer screening, prediction, and

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Biomarker Identification in Liver Cancers Using Desorption Electrospray Ionization Mass Spectrometrnging. Desorption electrospray ionization mass spectrometry (DESI-MS) imaging is a potent and sensitive MS ionization technique for direct, unaltered 2D and 3D imaging of metabolites in complex biological samples. Indeed, DESI gently desorbs and ionizes analyte molecules from the sample surface usin

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Kinetic Modeling of Hepatic Metabolism and Simulation of Treatment Effects,cular processes to cellular metabolic function. To this end, we developed detailed kinetic models of the central metabolism of different cell types, comprising multiple metabolic functionalities. We used the model to simulate metabolic changes in several cell types under different experimental setti

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Immunofluorescent Staining of Human Hepatic Multicellular Spheroids: A Model for Studying Liver Dis performing immunofluorescent staining and confocal imaging on whole human hepatic multicellular spheroids, utilizing primary cells or cell lines. The immunofluorescence technique is a potent tool to understand the spatial distribution of different cell types within the spheroids and define the interactions that occur among these cells.

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