Titlebook: Liquid Culture Systems for in vitro Plant Propagation; Anne Kathrine Hvoslef-Eide,Walter Preil Book 2005 Springer Science+Business Media B

Ataxia

Anne Kathrine Hvoslef-Eide,Odd Arild S. Olsen,Ragnhild Lyngved,Cristel Munster,Petter H. Heyerdahl

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Application of bioreactor design principles to plant micropropagation 30-litre air-lift and stirred bioreactors are used to illustrate design principles for circulation and mixing. Potential limitations to the use of liquid suspension culture due to plant physiological requirements are acknowledged.

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Multiplication of , shoots in bioreactors as affected by culture method and inoculation density of sFT culture led to the greatest fresh weight, shoot length and leaf area, followed by the ebb and flood culture, while the immersion culture suppressed shoot multiplication due to the lack of oxygen and the high water potential. Our results suggested the possibility of large-scale production of . sho

Scleroderma

Temporary immersion system: a new concept for use liquid medium in mass propagationto expose the plant to the liquid medium intermittently rather than continuously. For this the bioreactors previously developed are not suitable as they are mainly adapted to bacterial culture and do not take into account the specific requirements of plant cells and tissues, such as sensitivity to s

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Mass propagation of tropical crops in temporary immersion systemsnd examples are given to illustrate the different possibilities for TIS integration in propagation of .. and . for shoot multiplication and microtuber production. For sugarcane (. sp.) somatic embryo production in bioreactors, embryo germination in TIS and field performance of regenerated plants are

Throttle

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Somatic embryo germination of , L. in the Rita® temporary immersion system and on semisolid mediumemiliquid medium of the same composition (solidified with 2.5 g l. Gellum Gum, Spectrum®) in 250 ml glass vessels. The germination percentage, fresh weight and number of somatic embryos with complete germination were determined. After 10 weeks of culture the highest germination percentage (91%) and

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