Titlebook: Lipid Signaling Protocols; Mark G. Waugh Book 2016Latest edition Springer Science+Business Media New York 2016 cell function.ceramides.cho

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Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]Sol to measure ceramide and SM biosynthesis in cultured cells based on [.H]serine incorporation at the first step in the pathway. The method can be used to assay the effect of pharmacological and genetic manipulations on ceramide synthesis and transport to the Golgi apparatus.

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Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions,y allows measurement of the relative phosphoinositide composition of each membrane fraction or organelle and determination of the microenvironment in which each species is enriched. Here, we describe the steps to separate and quantify total or localized phosphoinositides from cultured cells.

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Single-Molecule Imaging of Signal Transduction via GPI-Anchored Receptors,may change the location of the target molecules. In this chapter, we describe how to examine dynamics of raft-associated glycosylphosphatidylinositol (GPI)-anchored receptors and interactions of the receptors with downstream signaling molecules by single-particle tracking or single-molecule imaging techniques.

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https://doi.org/10.1007/978-1-4939-3170-5cell function; ceramides; cholesterol-rich lipid rafts; detergent-resistant membrane; lipid signaling; me

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Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/586856.jpg

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Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary lar membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.

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