Titlebook: Lipid Signaling Protocols; Mark G. Waugh Book 2016Latest edition Springer Science+Business Media New York 2016 cell function.ceramides.cho

Fatuous

书目名称Lipid Signaling Protocols影响因子(影响力)




书目名称Lipid Signaling Protocols影响因子(影响力)学科排名




书目名称Lipid Signaling Protocols网络公开度




书目名称Lipid Signaling Protocols网络公开度学科排名




书目名称Lipid Signaling Protocols被引频次




书目名称Lipid Signaling Protocols被引频次学科排名




书目名称Lipid Signaling Protocols年度引用




书目名称Lipid Signaling Protocols年度引用学科排名




书目名称Lipid Signaling Protocols读者反馈




书目名称Lipid Signaling Protocols读者反馈学科排名

粘

,Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative Hilinositol-5-phosphate 4-kinase α (PI5P4Kα) utilizing the .-myo-di16-PtIns(5)P substrate in quantitative high-throughput screening (qHTS) format using the ADP-Glo™ technology to couple the production of ADP to a bioluminescent readout.

Physiatrist

olfction

Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-Kinasly identified actin cytoskeleton regulators—the WASH complex and RhoGEF1, as major novel interactors of PI4KIIα. While this chapter focuses on the PI4KIIα interactome, this protocol can and has been used to generate other membrane interactome networks.

AUGUR

性上瘾

,High-Throughput Fluorometric Assay for Membrane–Protein Interaction, rapid, sensitive, and accurate determination of lipid specificity and affinity for various lipid binding domains and proteins, and also high-throughput screening of small molecules that modulate membrane binding of proteins.

OTTER

1064-3745 ation advice from the experts.In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism

猜忌

Book 2016Latest editions techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. .Lipid Signaling Protocols, Second Edition. guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the t

大火

Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity,ll as for the identification of their ligands. In this chapter we describe in detail the assays performed to investigate LXR activity in a macrophage-like cell line (RAW 267.4). These can be easily adapted to other nuclear receptors and transcription factors.

micturition

,Using Surface Plasmon Resonance to Quantitatively Assess Lipid–Protein Interactions,ar interactions and the kinetics and affinity of an interaction. This technique has been especially useful in measuring the affinities of lipid-binding proteins to intact liposomes of varying lipid compositions.