Titlebook: Lipases and Phospholipases; Methods and Protocol Georgina Sandoval Book 2018Latest edition Springer Science+Business Media, LLC, part of Sp

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Functional Expression of Plant Lipases: The Case of CpLip1 from tly the first lipase from . latex (CpLip1) has been successfully cloned and expressed in order to study their catalytic properties. In order to improve the catalytic properties and increase the potential for its use at industrial scale..In this chapter, a practical protocol to recombinant CpLip1 lipase is given.

THROB

Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced FluoresPLD-generated PA and is not limited to PC as the substrate but allows the use of natural phospholipids with various headgroups. Besides, this easy-to-handle assay allows to monitor prokaryotic and eukaryotic PLD activities in a continuous way and on a microplate scale.

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BROOK

Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2 results corroborate with the fact that the catalytic activity of DaPLA., measured with the pH-stat using egg yolk as substrate, is mainly due to the hydrolysis of the PE fraction present in egg yolk, whereas the phosphatidylglycerol is a hallmark substrate for the most secreted PLA.-IB.

Angiogenesis

Expression and Purification of Recombinant , Phospholipase D in , for Structural Studiescowpea) into the yeast expression system . and its two-step purification process. This allowed us to purify to homogeneity hundreds of micrograms of highly pure protein to conduct . structural studies.

SSRIS

Carminative

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MIRE

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gonioscopy

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