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Titlebook: Lipases and Phospholipases; Methods and Protocol Georgina Sandoval Book 2018Latest edition Springer Science+Business Media, LLC, part of Sp

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发表于 2025-3-21 16:18:47 | 显示全部楼层 |阅读模式
书目名称Lipases and Phospholipases
副标题Methods and Protocol
编辑Georgina Sandoval
视频video
概述Includes cutting-edge methods and protocols for the study of lipases and phospholipases.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fro
丛书名称Methods in Molecular Biology
图书封面Titlebook: Lipases and Phospholipases; Methods and Protocol Georgina Sandoval Book 2018Latest edition Springer Science+Business Media, LLC, part of Sp
描述.This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful .Methods in Molecular Biology .series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and accessible, .Lipases and Phospholipases: Methods and Protocols, Second Edition.serves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes..
出版日期Book 2018Latest edition
关键词Expression systems; Plant lipases; Transcriptomic Analysis; Activity screening; Esterases; Enzymes; Biocat
版次2
doihttps://doi.org/10.1007/978-1-4939-8672-9
isbn_softcover978-1-4939-9361-1
isbn_ebook978-1-4939-8672-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
The information of publication is updating

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verbundenen Transaktionskosten zu untersuchen. Dazu wurde ein Modell entwickelt, in dem Verträge mit teilweise flexiblen Mengen durch Sicherheitsmaßnahmen abgesichert werden, die in verschiedenster Weise interpretiert werden können. Mögliche Interpretationen sind einige der im zweiten Kapitel der Ar
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Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substetrieved enzymes, because they are within the more demanded industrial enzymes and because a number of simple and generic screening methods can be applied for their screen. Here, we describe the use of a generic pH indicator assay protocol which unambiguously allows detecting in high-throughput mann
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A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities Using nd phospholipase A (PLA) activities. Here, a new ultraviolet spectrophotometric assay for lipase or PLA was developed using natural triglycerides or synthetic glycerophosphatidylcholines containing α-eleostearic acid (9., 11., 13.-octadecatrienoic acid) purified from . seed oil. The conjugated trien
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Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluores pathologies, such as cancer, making this enzyme an important therapeutic target. However, most PLD assays developed so far are either discontinuous or based on the indirect determination of choline released upon phosphatidylcholine (PC) hydrolysis. Therefore, we designed a PLD assay that is based o
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Lipase and Phospholipase Activity Methods for Marine Organismsmeasuring lipase and phospholipase activity have been applied in several studies; however, considering that lipases are water-soluble molecules and their substrates are generally water-insoluble molecules, several steps are required for measuring their digestion products. After a general review of t
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Functional Expression of Plant Lipases: The Case of CpLip1 from the lipolytic enzymes present in . latex. Therefore, diverse strategies have been developed to study the catalytic properties of these enzymes..Recently the first lipase from . latex (CpLip1) has been successfully cloned and expressed in order to study their catalytic properties. In order to improv
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