Titlebook: Light Microscopy; Methods and Protocol Yolanda Markaki,Hartmann Harz Book 2017 Springer Science+Business Media LLC 2017 confocal laser scan

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Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, ,contains several polymers that are highly similar to those found in the primary cell walls of land plants. . is easily grown in laboratory culture and is effectively manipulated in various experimental protocols including microplate assays and correlative microscopy. Most importantly, . can be live

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Targeted Ablation Using Laser Nanosurgerythis technique more precise, powerful, and easy to use. Today pulsed lasers can be operated with diffraction limited, sub-micrometer precision to ablate intracellular structures. Here, we discuss laser nanosurgery setups and the instrumentation in our laboratory. We describe how to use this techniqu

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STED Imaging in , Brain Slicesance, stimulated emission depletion (STED) microscopy has been successfully used in recent years to investigate the arrangement and colocalization of different protein species in cells in culture and on the surface of specimens. However, because of its extreme sensitivity to light scattering, super-

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Optical Coherence Microscopye review the functional imaging capabilities of OCM focusing on lable-free optical angiography. We conclude with a section on digital wavefront control and a short outlook on future developments, in particular for contrast enhancement techniques.

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Automated Analysis of Intracellular Dynamic Processesand therapies. Advanced fluorescence microscopy imaging systems nowadays allow the recording of virtually any type of process in space and time with super-resolved detail and with high sensitivity and specificity. The large volume and high information content of the resulting image data, and the des

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Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cellse gene of interest. The resulting reporter transgene is then integrated in the genome of cells that express the phage protein fused to a fluorescent tag. Upon transcription, binding of the fluorescent protein to its target sequence makes the RNA visible. With this approach it is possible to track, i

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