书目名称 | Light Microscopy |
副标题 | Methods and Protocol |
编辑 | Yolanda Markaki,Hartmann Harz |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imaging. .Light Microscopy: Methods and Protocols. puts special focus on system instrumentation parameters and sophisticated labeling and detection methods. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Light Microscopy: Methods and Protocols .offers the novice user with straightforward strategies to address biological questions, while providing the experienced researcher with the latest applications that can be useful in ro |
出版日期 | Book 2017 |
关键词 | confocal laser scanning and spinning disk confocal microscopy; Fluorescence Resonance Energy Transfer |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-6810-7 |
isbn_softcover | 978-1-4939-8305-6 |
isbn_ebook | 978-1-4939-6810-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media LLC 2017 |