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Titlebook: Light Microscopy; Methods and Protocol Yolanda Markaki,Hartmann Harz Book 2017 Springer Science+Business Media LLC 2017 confocal laser scan

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发表于 2025-3-21 16:43:54 | 显示全部楼层 |阅读模式
书目名称Light Microscopy
副标题Methods and Protocol
编辑Yolanda Markaki,Hartmann Harz
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Light Microscopy; Methods and Protocol Yolanda Markaki,Hartmann Harz Book 2017 Springer Science+Business Media LLC 2017 confocal laser scan
描述.This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imaging. .Light Microscopy: Methods and Protocols. puts special focus on system instrumentation parameters and sophisticated labeling and detection methods. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Light Microscopy: Methods and Protocols .offers the novice user with straightforward strategies to address biological questions, while providing the experienced researcher with the latest applications that can be useful in ro
出版日期Book 2017
关键词confocal laser scanning and spinning disk confocal microscopy; Fluorescence Resonance Energy Transfer
版次1
doihttps://doi.org/10.1007/978-1-4939-6810-7
isbn_softcover978-1-4939-8305-6
isbn_ebook978-1-4939-6810-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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发表于 2025-3-21 22:11:43 | 显示全部楼层
Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenere imaging, and multi-day window imaging. We carefully compare and explain in depth how to set up each method. Lastly, in the notes section we mention some alternative solutions for the 2P-IVM methods described. In conclusion, this protocol can be used as a guide towards deciding which 2P-IVM method to use and to enable the setup of this method.
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Book 2017sed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging f
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Introduction to Modern Methods in Light Microscopy,n of modern microscopy techniques. We briefly discuss the basics of optics ., super-resolution imaging, quantitative image analysis, live-cell imaging, and provide an outlook on active research areas pertaining to light microscopy.
发表于 2025-3-22 23:35:43 | 显示全部楼层
FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cellsted in intact cells or tissues and even in various subcellular compartments. Here, we describe how to perform FRET measurements in living cells expressing FRET-based biosensors and how to evaluate these data. This general protocol can be applied for FRET measurements with various fluorescent biosensors.
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