| 书目名称 | Leptospira spp. | | 副标题 | Methods and Protocol | | 编辑 | Nobuo Koizumi,Mathieu Picardeau | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume looks at all aspects of manipulation of .Leptospira spp.. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Leptospira spp.: Methods and Protocols. is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms. . | | 出版日期 | Book 2020 | | 关键词 | CRISPR; TALE; Chemotaxis assay; Biofilm; rat models; infectious diseases | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-0459-5 | | isbn_softcover | 978-1-0716-0461-8 | | isbn_ebook | 978-1-0716-0459-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |
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发表于 2025-3-21 16:26:14
