Titlebook: Leptospira spp.; Methods and Protocol Nobuo Koizumi,Mathieu Picardeau Book 2020 Springer Science+Business Media, LLC, part of Springer Natu

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In Situ Structural Analysis of , spp. by Electron Cryotomography,ation, data collection, tilt series alignment, and tomographic reconstruction for visualization of intact periplasmic flagella in . spp. This protocol is capable of determining protein structures at resolutions high enough to visualize their individual domains and secondary structures in their cellular environment.

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Core Genome Multi-locus Sequence Typing Analyses of , spp. Using the Bacterial Isolate Genome Sequeies, clades, clonal groups, and sequence types. We show several examples how the cgMLST . database, which is a publicly available web-based database, can be used for the analyses of WGS data of . isolates. This effort was undertaken in order to facilitate international collaborations and support the global surveillance of leptospirosis.

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1064-3745 ation advice from the experts.This volume looks at all aspects of manipulation of .Leptospira spp.. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from b

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