Titlebook: Leishmania; Methods and Protocol Joachim Clos Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Neglected Tropic

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Quantitative RNA Analysis Using RNA-Seq,ping the transcripts to the nucleotide level. Here we describe two different RNA-seq approaches, including one that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5′ end of all . (and other trypanosomatid) mRNAs.

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Cos-Seq: A High-Throughput Gain-of-Function Screen for Drug Resistance Studies in ,to improve knowledge about the mode of action of current drugs and those in development, as well as to understand the mechanisms pertaining to their resistance, we recently described a sensitive and high-throughput method termed Cos-Seq. Here we provide a detailed protocol for every step of the proc

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Gene Replacement by Homologous Recombination,that prove to be poor targets for CRISPR/cas-based approaches. Homologous recombination has proven to be relatively robust to minor sequence mismatches between GOI-flanking sequences and the gene replacement constructs, and the faithfulness of recombination events is easily verified by whole-genome

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