Titlebook: Kinase Signaling Networks; Aik-Choon Tan,Paul H. Huang Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 Protei

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https://doi.org/10.1007/978-1-4939-7154-1Protein science; Synthetic biology; Screening approaches; Mass spectrometry; Computational analysis; Chem

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Aik-Choon Tan,Paul H. HuangIncludes cutting-edge techniques for the study of kinase signaling networks.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts.Includes

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Book 2017ep-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Kinase Signaling Networks. aims to provide scientists with the tools necessary to overcome the technological bottlenecks that limit our ability to gain a deeper mechanistic understanding of kinase biology..

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Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensorsneeded to perform such measurements in a cell type of the investigator’s choosing. We discuss the selection of appropriate reporters and provide detailed methods for stably introducing reporters, collecting live-cell data, and automatically extracting quantitative information from individual cells.