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Titlebook: Kinase Signaling Networks; Aik-Choon Tan,Paul H. Huang Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 Protei

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书目名称Kinase Signaling Networks
编辑Aik-Choon Tan,Paul H. Huang
视频video
概述Includes cutting-edge techniques for the study of kinase signaling networks.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts.Includes
丛书名称Methods in Molecular Biology
图书封面Titlebook: Kinase Signaling Networks;  Aik-Choon Tan,Paul H. Huang Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 Protei
描述This detailed volume describes cutting-edge techniques in three distinct and complementary areas of contemporary kinase biology research. Beginning with a section on synthetic biology, chemical biology, and screening approaches to kinase signaling networks, the book continues with sections on mass spectrometry and metabolic analysis of kinase signaling as well as computational analysis of kinase signaling networks. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Kinase Signaling Networks. aims to provide scientists with the tools necessary to overcome the technological bottlenecks that limit our ability to gain a deeper mechanistic understanding of kinase biology..
出版日期Book 2017
关键词Protein science; Synthetic biology; Screening approaches; Mass spectrometry; Computational analysis; Chem
版次1
doihttps://doi.org/10.1007/978-1-4939-7154-1
isbn_softcover978-1-4939-8400-8
isbn_ebook978-1-4939-7154-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2017
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Utilizing the Luminex Magnetic Bead-Based Suspension Array for Rapid Multiplexed Phosphoprotein Quan-based xMAP. platform from Luminex. The system utilizes antibody-bound free-floating magnetic spheres which can easily be removed from suspension via magnetization. There are 100 unique bead sets (moving up to 500 bead sets for the most recent system) identified by the ratio of two dyes coating the
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High-Content Imaging and RNAi Screens for Investigating Kinase Network Plasticitywing cell fixation while also preserving the fluorescence of a stably expressed fluorescent protein-tagged reporter for CDK2 activity. We provide methodology for data extraction and handling including an R-script that converts the multidimensional data into four simple binary outcomes, on which a hi
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