Titlebook: Isolation of Plant Organelles and Structures; Methods and Protocol Nicolas L. Taylor,A. Harvey Millar Book 2017 Springer Science+Business M

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Wei Zeng,Berit Ebert,Harriet T. Parsons,Carsten Rautengarten,Antony Bacic,Joshua L. Heazlewood

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Anzu Minami,Daisuke Takahashi,Yukio Kawamura,Matsuo Uemura

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The Isolation of Plant Organelles and Structures in the Post-genomic Era, there is a great diversity of methods currently used for the initial physical disruption of plant tissue before the downstream isolation of a target cellular component. These include hand grinding, high-speed mechanical disruption, and enzymatic digestion of cell walls by a variety of methods. Coup

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Approaches to Characterize Organelle, Compartment, or Structure Purity,t topics in organelle research. To date, many organelle proteomics workflows involve the (partial) purification of the desired subcellular structure and the subsequent analysis of the proteome using tandem mass spectrometry (MS/MS). This chapter gives an overview of the methods that have been used t

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Isolation of Nuclei and Nucleoliadapted for other species and cell types. The resulting nuclei can be further purified for use in biochemical or proteomic studies, or can be used for microscopy. We also describe how the nuclei can be used to obtain a preparation of nucleoli.

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Isolation and Suborganellar Fractionation of Arabidopsis Chloroplastsnner aqueous compartment called the stroma, and possess a second internal membrane system called the thylakoids. Thus, determining the suborganellar location of a chloroplast protein is vital to understanding or verifying its function. One way in which protein localization can be addressed is throug

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