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Titlebook: Isolation of Plant Organelles and Structures; Methods and Protocol Nicolas L. Taylor,A. Harvey Millar Book 2017 Springer Science+Business M

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发表于 2025-3-21 19:56:22 | 显示全部楼层 |阅读模式
书目名称Isolation of Plant Organelles and Structures
副标题Methods and Protocol
编辑Nicolas L. Taylor,A. Harvey Millar
视频video
概述Includes cutting-edge methods and protocols for the study of plant organelles and structures.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advic
丛书名称Methods in Molecular Biology
图书封面Titlebook: Isolation of Plant Organelles and Structures; Methods and Protocol Nicolas L. Taylor,A. Harvey Millar Book 2017 Springer Science+Business M
描述This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful .Methods in Molecular Biology. series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Isolation of Plant Organelles and Structures: Methods and Protocols. will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study..
出版日期Book 2017
关键词Subcellular structures; Plant cells; Organelle compartments; Cellular components; Chloroplasts
版次1
doihttps://doi.org/10.1007/978-1-4939-6533-5
isbn_softcover978-1-4939-8228-8
isbn_ebook978-1-4939-6533-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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发表于 2025-3-21 20:37:44 | 显示全部楼层
Isolation of Plasma Membrane and Plasma Membrane Microdomainsusters are called as “microdomains.” In plant cells, microdomains have been studied extensively because they play important roles in biotic/abiotic stress responses, cellular trafficking, and cell wall metabolism. Here we describe a standard protocol for the isolation of the plasma membrane and microdomains from plant cells, . and oat.
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Isolation of Vacuoles and the Tonoplastroteins involved in stress responses. In addition, vacuolar lumen preparations have also allowed characterization of a luminal solute content in response to various abiotic stresses. Here, I revisit and update one of the most successful methodologies for vacuole and tonoplast isolation.
发表于 2025-3-22 12:38:50 | 显示全部楼层
Isolation of Endoplasmic Reticulum and Its Membrane conjunction with equilibrium density gradient centrifugation as a means to diagnose whether a protein is associated with the ER. The same approach can also be used as a means to enrich for ER membranes.
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Isolation and Suborganellar Fractionation of Arabidopsis Chloroplastseparation of isolated chloroplasts into three suborganellar fractions (envelope membranes, a soluble fraction containing stromal proteins, and the thylakoids). These methods are routinely used in our laboratory, and they provide a good yield of isolated chloroplasts and suborganellar fractions that can be used for various downstream applications.
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Isolation of the Cell Wallexplain why its study as a true cell compartment is of high interest. The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material. Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.
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