Titlebook: Isolation of Plant Organelles and Structures; Methods and Protocol Nicolas L. Taylor,A. Harvey Millar Book 2017 Springer Science+Business M

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Isolation of Chromoplasts and Suborganellar Compartments from Tomato and Bell Pepper Fruitugations. It yields high purity organelles suitable for proteome analyses. Enzymatic and microscopy assays are summarized to assess purity and intactness. A method is also described for subfractionation of pepper chromoplast lipoprotein structures.

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Isolation of Autolysosomes from Tobacco BY-2 Cellses. It contains acid phosphatase, vacuolar H.-ATPase, and protease activity. Electron micrographs show that the fraction contains partially degraded cytoplasm seen in autolysosomes before isolation although an autolysosome structure is only partially preserved.

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1064-3745 oducible results.Contains key notes and implementation advicThis book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the

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Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membraner the isolation of Arabidopsis leaf peroxisomes using two different types of density gradients (Percoll and sucrose) sequentially that yields approximately 120 μg of peroxisome proteins from 60 g of fresh leaf material. A method is also provided to assess the relative purity of the isolated peroxiso

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Stefanie J. Mueller,Sebastian N. W. Hoernstein,Ralf Reski