Titlebook: Investigation of Membrane-Located Receptors; Eric Reid,G. M. W. Cook,D. J. Morré Book 1984 Springer Science+Business Media New York 1984 G

DAUNT

Determination of Molecular Weights of Receptors in Solution and ,thods of protein physical chemistry need important modifications to be applicable to receptors, due to the effects of the receptor-hound detergent in the solutions used. Rigorous methods are available now for this, and should be applied. Subunit analyses can also be used, with certain precautions. D

outset

Lipoprotein(A)

Approaches to Isolating Minor Membrane Components Using Monoclonal Antibodies, as in Purifying 5′-Nugood purity and yield. Amphi-pathic detergents of diverse molecular structure are commonly used, aiming at “the smallest and least polydisperse active protein” [1] by criteria including non-sedimentability at high g forces and Polyacrylamide gel electrophoresis (PAGE) which also indicates the solubi

用不完

Isolation of Purified Membranes and Membranous Cell Components for Receptor Studiescterization, biosynthesis, turnover and function of membrane-located receptors. Procedures are described that yield purified preparations of Golgi apparatus (G.a.), endoplasmic reticulum (e.r.J, plasma membrane (p.m.), mitochondria and nuclei from liver tissue; advantageously, the procedures can be

Kidney-Failure

Molecular Genetics Applied to Receptor Analysis’s coding for the receptor protein can be obtained by cloning technology, sequencing of the DNA is completely feasible and very rapid, and the amino acid sequences of the subunits can at once be deduced by means of the genetic code. In view of the vanishingly small amounts of protein that can be obt

mendacity

Isolation and Characterization of Spiny- (Clathrin)-Coated Membranes and Vesicles from Rodent Liver or-mediated endocytosis and secretion. Clathrin is the major coat protein of both endocytotic and exocytotic vesicles and membranes in this and other tissues, and presumably plays some yet unknown role in membrane translocations.

错误

Hormone Receptor — Adenylate Cyclase Interaction: Superpotency, Superaffinity and Desensitization Phf agonists such as protein hormones are joined to a large rigid carrier molecule such as a virus, they may exhibit superpotency that reflects superaffinity. (3) Catecholamine desensitization as studied with turkey erythrocytes is due to a regulatory defect at the level of nucleotide regulatory prote

CHARM

prostatitis

Architecture of Plasma Membrane Hormone Receptor-Effector Systemsing of components to one another and to elements of the cytoskeleton. In these and other interactions such as those with specific membrane lipids, cytosolic macromolecules, and metabolites, adenylate cyclase undergoes reversible changes in catalytic activity. The associative and dissociative process

细微差别

Epithelial Sodium Channels: Ligand Binding Techniques in Intact Tissues and Homogenatesivative (labeled), [.H]benzamil, the choice of which is considered, binding studies have been performed under optimized conditions with intact epithelia and with homogenates. Related work, by current fluctuation analysis has been done elsewhere. Data are presented from experiments with frog skin epi